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KMID : 0613820080180050646
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Journal of Life Science
2008 Volume.18 No. 5 p.646 ~ p.652
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Biochemical Properties of EmeA, a Multidrug Efflux Pump, from Enterococcus faecalis
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Kwon Hyun-Ju
Kim Byung-Woo
Kim Kwang-Hyeon
Kim Young-Hee
Lee Eun-Woo
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Abstract
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It was reported that expression of emeA gene, which was cloned from Enterococcus faecalis ATCC 29212, is responsible for the resistance against several antibiotics and for the efflux drugs, like as DAPI and Hoechst 33342. In this study, emeA gene was expressed in Escherichia coli KAM32, which is hypersensitive to many antibiotics and antimicrobial agents, and the biochemical properties of EmeA, a multidrug efflux pump, were investigated. EmeA showed 12 transmembrane domain structure which was constituted with 393 amino acids. EmeA showed significant homology in amino acid sequence with Bit of Lactococcus lactis, NorA of Staphylococcus aureus, and TetA of E. coli. EmeA expressing cells showed significantly lower accumulation level of ethidium bromide and acriflavine than EmeA non-expressing cells. Moreover, EmeA expressing cells represented efflux activity of ethidium bromide and acriflavine from the cells in an energy-dependent manner. This efflux activity was strongly inhibited by reserpine, which was known as a strong inhibitor of many drug efflux pumps.
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KEYWORD
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Enterococcus faecalis, multidrug efflux pump, antibiotic resistance, EmeA
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